WSU

Devin M. Drown

Ph.D. Candidate

cougar

Protocols

Dybdahl Lab Protocols

DNA extraction for snails
PCR for cyt b for snails
PCR for microsats
Gel documentation and running
SAP/Exo cleanup
Cycle Sequencing

Dybdahl Lab Datasheets

DNA Extraction datasheet
PCR datasheet
Sequencing datasheet

Unauthorized HOWTOs

Detailed protocols from Sample to Sequence (well almost)
These are taken from the Barber lab where I used to work.

DNA Extraction via quick and cheap chelex beads
PCR using standard "hot start" conditions
Agarose gel electrophoresis for visualizing PCR fragments
SAP/Exo cleanup to get PCR product ready for sequencing
Cycle Sequencing to get DNA sequence data

Contact Info

School of Biological Sciences
Washington State University
Pullman, WA 99164-4236
509-335-7914
drown(at)wsu.edu

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