Protocols

Dybdahl Lab Protocols

• DNA extraction for snails
• PCR for cyt b for snails
• PCR for microsats
• Gel documentation and running
• SAP/Exo cleanup
• Cycle Sequencing

Dybdahl Lab Datasheets

• DNA Extraction datasheet
• PCR datasheet
• Sequencing datasheet

Unauthorized HOWTOs

• Arlequin
• Treemap

Detailed protocols from Sample to Sequence (well almost)

These are taken from the Barber lab where I used to work.

• DNA Extraction via quick and cheap chelex beads
• PCR using standard "hot start" conditions
• Agarose gel electrophoresis for visualizing PCR fragments
• SAP/Exo cleanup to get PCR product ready for sequencing
• Cycle Sequencing to get DNA sequence data