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Dmitri
Mavrodi
Assistant Scientist
B.S. Kuban State University, Krasnodar, Russia 1992
Ph.D. Institute of Molecular Biology, Moscow, Russia 1997
Office
349 Johnson Hall
Tel.: (509)335-3269
Fax: (509)335-7674
E-mail:
mavrodi@mail.wsu.edu
Professional Experience:
Assistant Scientist, Department of Plant Pathology, Washington State University, 07/99-present.
Research Scientist, Department of Microbial Genetics, Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences, Pushchino, Russia, 08/00-11/01.
Postdoctoral Research Fellow with Dr. Linda Thomashow. USDA-ARS Root Disease and Biocontrol Unit, Department of Plant Pathology, Washington State University. 02/97-07/99.
Junior Research Scientist, Department of Microbial Genetics, Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences, Pushchino, Russia, 11/95-02/97.
Graduate work, Molecular Biology, Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences, Pushchino, Russia. 11/92-11/95. Advisor: Prof. Alexander M. Boronin, Dr. Vladimir N. Ksenzenko. Thesis: Structural and Functional Study of Genes for Biosynthesis of Phenazine Antibiotics in Rhizosphere Pseudomonads.
Undergraduate work, Department of Genetics and Microbiology, Kuban State University, Krasnodar, Russia. 09/87-09/92.Advisors: Prof. Boris I. Rukavtsov, Dr. Tamara V. Tsoi.Undergraduate Thesis: Genomic Library of Corynebacterium sepedonicum: Construction and Screening.
Research Specialty and Interests: Genetics and enzymology of antibiotic production; mechanisms of gene regulation and expression; ecology and diversity of antibiotic-producing pseudomonads; plant growth-promoting rhizobacteria.
Teaching Responsibilities: Advising graduate students in the laboratory.
Memberships: American Phytopathological Society (APS) (St. Paul, MN); International Society for Molecular Plant-Microbe Interactions (IS-MPMI) (St. Paul, MN)
Professional
Activities:
Ad hoc reviewer for: Molecular Microbiology, Journal of Bacteriology, Applied
and Environmental Microbiology, Letters in Applied Microbiology, Plant and
Soil, Soil Biology and Biochemistry, Chemical Reviews, Journal of Applied
Microbiology, Canadian Journal of Microbiology, FEMS
Microbiology Letters
Selected Publications:
Validov S., O. Mavrodi, L. De La Fuente, A. Boronin, D. Weller, L.Thomashow, and D. Mavrodi. 2005. Antagonistic activity among 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. FEMS Microbiol. Lett. 242:249-256.
Blankenfeldt, W., A. P. Kuzin, T. Skarina, Y. Korniyenko, L. Tong, P. Bayer, P. Janning, L. S. Thomashow, and D. V. Mavrodi. 2004. Structure and function of phenazine biosynthesis protein PhzF from Pseudomonas fluorescens 2-79. Proc. Natl. Acad. Sci. U.S.A. 101:16431-16436.
Lau, G.W., H. Ran, F. Kong, D. J. Hassett, and Mavrodi, D.V. 2004. Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice. Infection and Immunity. 72:4275-4278.
Huang Z., Bonsall,R.F., Mavrodi, D.V.,, Weller, D.M., and Thomashow, L.S. 2004. Transformation of Pseudomonas fluorescens with genes for biosynthesis of phenazine-1-carboxylic acid improves biocontrol activity and in situ antibiotic production.FEMS Microbiology Ecology 49:243-251.
Ahuja, E. G., Mavrodi, D., Thomashow, L., and Blankenfeldt, W. 2004. Overexpression, purification and crystallisation of PhzA, the first enzyme of the phenazine biosynthesis pathway of Pseudomonas fluorescens 2-79. Acta Crystallographica. D60:1129-1131.
Mavrodi D.V., N. Bleimling, L. S.Thomashow, and W. Blankenfeldt. 2004. The purification, crystallisation and preliminary structural characterisation of PhzF, a key enzyme in the phenazine biosynthesis pathway from Pseudomonas fluorescens 2-79. Acta Crystallographica. D60:184-186.
Mavrodi D.V., N. P. Kovalenko, S. L. Sokolov, V. G. Parfenyuk, I.A.Kosheleva, and A.M.Boronin. 2003. Identification of key genes of naphthalene catabolism in soil DNA by polymerase chain reaction. Microbiology (Moscow) 72: 597-604 (in Russian).
Landa, B. B., D. V. Mavrodi, L. S. Thomashow, and D. M. Weller. 2003. Interactions between strains of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens in the rhizosphere of wheat. Phytopathology 93(8): 982-994.
Mavrodi, D. V., O. V. Mavrodi, B. B. McSpadden-Gardener, B. B. Landa, D. M. Weller, and L. S. Thomashow. 2002. Identification of differences in genome content among phlD-positive Pseudomonas fluorescens strains by using PCR-based subtractive hybridization. Appl. Environ. Microbiol. 68(10):5170-5176.
Mavrodi, D. V., R. F. Bonsall, S. M. Delaney, M. J. Soule, G. Phillips, and L. S. Thomashow. 2001. Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PA01. J. Bacteriol. 183(21): 6454-6465.
McDonald, M., D. V. Mavrodi, L. S. Tomashow, and H. G. Floss. 2001. Phenazine Biosynthesis in Pseudomonas fluorescens: Branchpoint from the Primary Shikimate Biosynthetic Pathway and Role of Phenazine-1,6-dicarboxylic Acid. The Journal of the American Chemical Society 123:9459-9460.
Delaney, S. M., D. V. Mavrodi, R. F. Bonsall, and L. S. Thomashow. 2001. phzO, a gene for biosynthesis of 2-hydroxylated phenazine compounds in Pseudomonas aureofaciens 30-84. J. Bacteriol. 183:318-327.
McSpadden Gardener, B. B., D. V. Mavrodi, R. F. Bonsall, L. S. Thomashow, and D. M. Weller. 2001. A rapid polymerase chain-based assay characterizing rhizosphere populations of 2,4-diacetylphloroglucinol-producing bacteria. Phytopathology 91:44-54.
Mavrodi, O. V., B. B. McSpadden Gardener, D. V. Mavrodi, L. S. Thomashow, and D. M. Weller. 2001. Genetic diversity of phlD from 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. Phytopathology 91:35-43.
Timms-Wilson, T. M., R. J. Ellis, A. Renwick, D. J. Rhodes, D. V. Mavrodi, D. M. Weller, L. S. Thomashow, and M. J. Bailey. 2000. Chromosomal insertion of phenazine-1-carboxylic acid biosynthetic pathway enhances efficacy of damping-off control by Pseudomonas fluorescens. Mol. Plant-Microbe Interact. 13:1293-1300.
Thomashow, L. S., O. V. Mavrodi, D. V. Mavrodi, J. M. Raaijmakers, R. F. Bonsall, B. McSpadden-Gardener, Y.-J. Lee, M. J. Park, and D. M . Weller. 2000. 2,4-diacetylphloroglucinol, a major determinant in the biological control of take-all disease of wheat, p. 331-335. In: P.G.M. De Witt, T. Bisseling, and W. J. Stiekema (ed.). Biology of Plant Microbe Interactions, Vol. 2. International Society for Molecular Plant-Microbe Interactions, St. Paul, MN.
Sokolov, S. L., I. A. Kosheleva, O. V. Mavrodi, D. V. Mavrodi, and A. M. Boronin. 1998. Structural organization and expression of the salicylate hydroxylase gene in the Pseudomonas putida BS814 (pBS106) strain. Russian Journal of Genetics. 34:206-212 (in Russian).
Mavrodi, D. V., V. N. Ksenzenko, R. E. Bonsall, R. J. Cook, A. M. Boronin, and L. S. Thomashow. 1998. A seven-gene locus for synthesis of phenazine-1-carboxylic acid by Pseudomonas fluorescens 2-79. J. Bacteriol. 180: 2541-2548.
Mavrodi, D. V., V. N. Ksenzenko, B. M. Chatuev, L. S. Thomashow, and A. M. Boronin. 1997. Structure-functional organization of Pseudomonas fluorescens genes, coding for biosynthetic enzymes of phenazine-1-carboxylic acid by 2-79. Mol. Biology (Moscow). 31 (1): 74-80 (in Russian).
Patents:
US Patent 6,737,260. Thomashow, D. M. Weller, D. V. Mavrodi, Delaney, S. M. Sequences Encoding PhzO and Methods. Issued: May 18, 2004
US Patent 6,277,625. Huang, Z., L. S. Thomashow, D. V. Mavrodi, J. M. Raaijmakers, D. M. Weller, R. J. Cook. Transgenic strains of Pseudomonas for biocontrol of plant root diseases. Issued: August 21, 2001
Olga
Mavrodi
Postdoctoral
Research Associate
Ph.D., Washington State University, Pullman, WA 2004
M.S., Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russia 1996
B.S. Kuban State University, Krasnodar, Russia 1992
362 Johnson Hall
Tel.: (509)335-3269
Fax: (509)335-7674
E-mail: omavrodi@mail.wsu.edu
Professional Experience:
Postdoctoral Research Associate, Department of Plant Pathology, Washington State University, Pullman, WA 2005-current.
Associate in Research, Department of Plant Pathology and USDA-ARS Root Disease and Biocontrol Research Unit, Washington State University, Pullman, WA 1997-1999.
Research Technician, Laboratory of Plasmid Biology, Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences, Pushchino, Russia, 1992-1997
Technician, Department of Hospital Surgery, Kuban Medical Institute, Krasnodar, Russia, 1992.
Research Specialty and Interests: Phenotypic, genotypic, and colonization properties of 2,4-diacetylphloroglocinol-producing. Pseudomonas strains isolated from different crops and soils.
Publications:
Mavrodi, O. V., B. B. McSpadden Gardener, D. V. Mavrodi, L. S. Thomashow, and D. M. Weller. 2001. Genetic diversity of phlD from 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. Phytopathology 91:35-43.
Sokolov, S. L., I. A. Kosheleva, O. V. Mavrodi, D. V. Mavrodi, and A. M. Boronin. 1998. Structural organization and expression of the salicylate hydroxylase gene in the Pseudomonas putida BS814 (pBS106) strain. Russian Journal of Genetics. 34:206-212 (in Russian).
Karen
Hansen
Biological Research Technician
B.S. Utah State University, 1989
362 Johnson Hall
Tel.: (509)335-3269
Fax: (509)335-7674
E-mail: kmhansen@wsunix.wsu.edu
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