Genetics 301, Spring 2003
Homework Assignment #9
You are interested in cloning and sequencing the genome of a bacteria that grows in hot springs in Yellowstone National Park. The genome size is about 5 MB (million bases).
- If the average size of BAC inserts is 150 kb, how many BACs would be needed to cover the genome?
- If the inserts on average had 50% overlap with other BACs (to allow construction of Contigs) how many inserts would be needed?
- How might you go about using restriction enzymes for generating the large inserts used in such a library? What enzymes (e.g., 4, 6 or 8 base recognition) might you choose, and why? Would a partial digestion with another type of enzyme be a viable option?
- Describe at least two ways in which you might interpret the data as you sequence the genome.
What results might you obtain if you used a cDNA clone (insert 1.8 kb in size) to probe a BAC (genomic) library (average insert size 150 kb), and what additional information might you obtain from analyzing the BAC clone(s) that you isolate?
Also, problems 10.3 (note: tet stands for the antibiotic tetracycline), 10.4, 10.7, 10.8 and 11.3