| Determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat Theor. Appl.Genet. 109:1597-1603 |
Gedye, K.R., C.F. Morris and A.D. Bettge
Dept. Food Sci and Human Nutrition, Washington State University
USDA-ARS Western Wheat Quality Lab, Pullman, WA |
| Aegilops tauschii (2n=2 x=14, DD) is a rich
source of genetic variability for hexaploid wheat (Triticum
aestivum, 2n=6 x=42, AABBDD) improvement. This
variability can be accessed through utilizing synthetic
hexaploid wheat lines, which contain genomes from Ae.
tauschii and T. turgidum (2n=4 x=28, AABB). Numerous
desirable characteristics can and have been introgressed
into common hexaploid wheat with this germplasm. In this
work, the genetic variability in the two puroindoline genes
(a and b) contained on the D genome, and the relationship
that sequence polymorphisms in these genes have on
endosperm texture among a population of 75 CIMMYT
synthetic hexaploid accessions is described. Kernel texture
was evaluated using the single kernel characterization
system (SKCS). Kernel texture differed significantly
(P=0.0001) among the synthetic hexaploid accessions
(range 2.6–40.9) and the parent types, durum or Ae.
tauschii. The interaction term between parent types was
also a significant effect (P=0.0001). In addition to the
‘wild-type’ protein sequences of the puroindoline genes
(those present in ‘Chinese Spring’ and all other soft
wheats), three other translated sequences were identified in
puroindoline a and two others in puroindoline b. These
protein sequences were associated with significantly
(P=0.0001) softer endosperm textures than the wild-type
protein sequences. As the softer alleles are expressed in a
hexaploid background, they are immediately available to
wheat breeding programs.
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