(Portrait)

M.K.(Kay) Walker-Simmons

National Program Leader, Grain Crops



USDA/ARS/NPS, 5601 Sunnyside Ave.,
Room 4-2230,
Beltsville, MD 20705-5139
Phone: 301-504-5560
FAX: 301-504-6191
Cell: 301-675-9331
kws@ars.usda.gov

PUBLICATIONS SINCE 1990

1999

Gomez-Cadenas A, Verhey SD, Holappa LD, Shen Q, Ho T-H D, Walker-Simmons MK (1999) An abscisic acid-induced protein kinase, PKABA1, mediates abscisic acid-suppressed gene expression in barley aleurone layers. Proc. Natl. Acad. Sci. USA 99:1767-1772.

Donoghue AM and Walker-Simmons MK (1999) The influence of wheat dehydration-induced proteins on the function of turkey spermatozoa after twenty-four-hour in vitro storage. Poultry Science 78:235-241.

Walker-Simmons MK, Rose PA, Hogge LR and Abrams SR (1999) Abscisic acid immunoassay and gas chromatography/mass spectrometry verification. In: J.Roberts & G. Tucker, eds., Plant Hormones, Methods in Molecular Biology Series, Humana Press, Inc. Totowa, NJ. In press.

1998

Storlie EW, Allan RE and Walker-Simmons MK (1998) Effect of the Vrn1-Fr1 interval on cold hardiness levels in wheat near-isogenic lines. Crop Science 38:483-488.

Walker-Simmons, M.K. (1998) Protein kinases in seeds. Seed Science Research 7:125-134.

Storlie EW, Walker-Simmons MK, Anderson JA and Allan RE (1998) Effect of the Fr1-Vrn1 interval on cold hardiness in wheat. Proceedings of the 9th International Wheat Genetics Symposium, Vol. 4, pp. 92-94.

1997

Goldmark PJ, Wiens JD, Verhey SD, Walker-Simmons MK (1997) Nucleotide sequence of a cDNA clone for an oleosin from dormant Bromus secalinus seeds (accession no. U72411) (PGR 97-002). Plant Physiol. 113:305.

Verhey SD and Walker-Simmons MK (1997) Abscisic acid-mediated responses in seeds involving protein kinases and phosphatases. In: Basic and Applied Aspects of Seed Biology, eds. R.E. Ellis, M. Black, A.J. Murdoch, and T.D. Hong, Kluwer Academic Publishers, Dordrecht, pp. 225-233.

Holappa LD, Walker-Simmons MK (1997) The wheat protein kinase gene, TaPK3, of the PKABA1 subfamily is differentially regulated in greening wheat seedlings. Plant Molecular Biology 33:935-941.

Abrams SR, Rose PA, Cutler AJ, Balsevich JJ, Lei B, and Walker-Simmons MK (1997) 8'-Methylene ABA - An effective and persistent analog of abscisic acid. Plant Physiol. 114:89-97.

Walters C, Ried JL, and Walker-Simmons MK (1997) Hydration characteristics of the heat soluble proteins from mature wheat embryos. Seed Science Research 7:125-134.

Walker-Simmons MK, Holappa Lynn D, Abrams Garth D and Abrams Suzanne R (1997) ABA metabolites induce group 3 LEA mRNA and inhibit germination in wheat. Physiol. Plant. 7:125-134.

1996

Walker-Simmons MK and Holappa LD (1996) An abscisic acid-regulated protein kinase expressed in dormant wheat seed embryos. In: Seventh International Symposium on Pre-Harvest Sprouting in Cereals 1995, eds. K. Noda and D.J. Mares, Center for Academic Societies, Osaka, Japan, pp. 117-121.

Abrams, SR, Rose PA, and Walker-Simmons MK (1996) Structural requirements of the ABA molecule for maintenance of dormancy in excised wheat embryos. Plant Dormancy. 1996, ed. G. Lang, CAB International, Oxon, UK, pp. 213-231.

Walker-Simmons MK and Goldmark PJ (1996) Characterization of genes expressed when dormant seeds of cereals and wild grasses are hydrated and remain growth-arrested. In: Plant Dormancy. 1996, ed. G. Lang, CAB International, Oxon, UK, pp. 283-291.

Rose PA, Lei B, Shaw AC, Barton DL, Walker-Simmons MK, and Abrams SR (1996) Probing the role of the hydroxyl group of ABA: Analogues with a methyl ether at C-1'. Phytochem: 41:1251-1258.

Rose PA, Lei B, Shaw AC, Walker-Simmons MK, Napper S, Quail JW, and Abrams SR (1996) Chiral synthesis of (+)-8'-demethyl abscisic acid. Can J Chem 74:1836-1843.

1995

Holappa, L.D., and Walker-Simmons, M.K. (1995) A wheat ABA-responsive protein kinase mRNA, PKABA1, is upregulated by dehydration, cold temperature and osmotic stress. Plant Physiology: 108:1203-1210.

1994

Cadle, M.M., Rayfuse, L.M., Walker-Simmons, M.K. and Jones, S.S. (1994) Mapping of ABA-responsive genes and vp1 to chromosomes in wheat and Lophopyrum elongatum. Genome 37:129-132, 1994.

Farmer, E.E., Caldelari, D., Pearce, G., Walker-Simmons, M.K., and Ryan, C.A. (1994) Diethyldthiocarbamic acid (DIECA) inhibits the octadecanoid signalling pathway for the wound induction of proteinase inhibitors in tomato leaves. Plant Physiology 106:337-342.

Walker-Simmons, M.K., Rose, P.A., Shaw, A.C. and Abrams, S.R. (1994) The 7'-methyl group of ABA is critical for biological activity in wheat embryo germination. Plant Physiology: 106:1279-1284.

1993

Curry, J. and Walker-Simmons, M.K. (1993) Unusual sequence of group 3 LEA (II) mRNA inducible by dehydration stress in wheat. Plant Mol. Biol. 21:907-912.

Ried, J.L. and Walker-Simmons, M.K. (1993) Group 3 late embryogenesis abundant proteins in desiccation-tolerant seedlings of wheat. Plant Physiol. 102:125-131.

Rayfuse, L.M., Cadle, M.M., Goldmark, P.J. Anderberg, R.J., Walker-Simmons, M.K. and Jones, S.S. (1993) Chromosome location and linkage relations of seven genes associated with seed dormancy. In PreHarvest Sprouting 1992, eds. M.K. Walker-Simmons and J.L. Ried, AACC Press, St. Paul, MN, pp. 129-135.

Goldmark, P. J., and Walker-Simmons, M.K. (1993) Expression of a Bromus secalinus transcript associated with seed dormancy in Avena fatua and other grass weeds. In Pre-Harvest Sprouting 1992, eds. M.K. Walker-Simmons and J.L. Ried, AACC Press, Minneapolis, MN, pp. 312-316.

Pre-Harvest Sprouting in Cereals 1992, (1993), editors M.K. Walker-Simmons and J.L. Ried, American Association of Cereal Chemistry Press, St. Paul, MN, 480 pp.

Curry, J. and Walker-Simmons, M.K. (1993) Sequence analysis of wheat cDNAs for ABA-responsive genes expressed in dehydrated wheat seedlings and the cyanobacterium, Anabaena. In Plant Responses to Cellular Dehydration during Environmental Stress, eds. T.J. Close and E.A. Bray, American Soc. of Plant Physiologists, Rockville, MD, pp. 128-136.

1992

Walker-Simmons, M.K., Anderberg, R.J., Rose, P.A., and Abrams, S.R. (1992) Optically pure ABA analogs - Tools for relating germination inhibition and gene expression in wheat embryos. Plant Physiol. 99: 501-507.

Ried, J.L., Everard, J.D., Diani, J., Loescher, W.H., and Walker-Simmons, M.K. (1992) Production of polyclonal antibodies in rabbits is simplified using perforated plastic golf balls. BioTechniques 12: 661-666.

Goldmark, P.J., Curry, J., Morris, C.F., and Walker-Simmons, M.K. (1992) Cloning and expression of an embryo-specific mRNA up-regulated in hydrated dormant seeds. Plant Molecular Biology 19: 433-441.

Anderberg, R.J. and Walker-Simmons, M.K. (1992) Isolation of a wheat cDNA clone for an abscisic acid-inducible transcript with homology to protein kinases. Proc. Natl. Acad. Sci. USA 89: 10183-10187.

1991

Curry, J., Morris, C.F., and Walker-Simmons, M.K. (1991) Sequence analysis of a cDNA encoding a Group 3 LEA mRNA inducible by ABA or dehydration stress in wheat. Plant Mol. Biol. 16:1073-1076.

Morris, C., Anderberg, R.A., Goldmark, P.J., and Walker-Simmons, M. (1991) Molecular cloning and expression of abscisic acid-responsive genes in embryos of dormant wheat seeds. Plant Physiol. 95:814-821.

Walker-Simmons, M., Reaney, Quarrie, S.A., Perata, P., Vernieri, P., and Abrams, S.R. (1991) Monoclonal antibody recognition of abscisic acid analogs. Plant Physiol. 95: 46-51.

Walker-Simmons, M.K. and Abrams, S.R. (1991) Uses of ABA Immunoassays. In: W.J. Davies and H.G. Jones, Abscisic Acid - Physiology and Biochemistry, Bios, Oxford, U.K., pp. 53-61.

1990

Ried, J.L. and Walker-Simmons, M. (1990) Synthesis of abscisic acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain. Plant Physiol. 93:662-667.

Walker-Simmons, M., Chandler, P.M., Sesing, J., Ried, J.L. and Morris, C.F. (1990) "Comparison of responses to ABA in embryos and dehydrating seedlings of 46-51. sprouting resistant and susceptible wheat cultivars, eds, K. Ringlund, E. Mosleth and D. Mares, Fifth International Symposium on Preharvest Sprouting, Westview Press, pp. 119-129.

Walker-Simmons, M. and Sesing, J. (1990) Temperature effects on embryonic ABA levels during development of wheat grain dormancy. J. of Plant Growth Regulation 9:51-56.

Hill, R.D., Walker-Simmons, M., Robertson, M. (1990) Effects of the environment and ABA on the synthesis of -amylase inhibitor. In (K. Ringlund ed.) Fifth International Symposium on Preharvest Sprouting, Westview Press: Bolder, CO.

Walker-Simmons, M. (1990) Abscisic acid. In (S.P. Parker, ed.) McGraw-Hill Yearbook of Science and Technology 1991, McGraw-Hill: New York, pp. 1-3.

Walker-Simmons, M. (1990) Dormancy in cereals - Levels and response to abscisic acid. In (R.P. Pharis and S.B. Rood, eds.) Plant Growth Substances 1988, Springer-Verlag: Berlin, pp. 400-406.

Walker-Simmons, M., Crane, K.E., and Yao, S. (1990). Synthesis of acid-soluble, ABA-inducible proteins in wheat embryos from dormant grain. In: R. Taylorson, ed., Recent Advances in Development and Germination of Seeds., Plenum Publ. Co., NY., pp. 47-55.

EDUCATION

Ph.D., Biochemistry, University of California, Los Angeles, 1974
B.A., Chemistry, Catholic University of America, 1969